Cultured Transformed Human Podocytes

These podocytes are obtained from human patients and have been transformed by laboratory viruses so that they are immortal.  The SV40 T antigen that is used to transform the cells is temperature-sensitive and so at 37˚C it is not active. The cells proliferate at 33 C and differentiate at 37 C. Cells from individuals with and without APOL1 renal risk genetic variants are available.