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  5. Detection of nucleic acid sequence variations using phase Mu transposase (U.S. Patent Number 7,316,903)

Detection of nucleic acid sequence variations using phase Mu transposase (U.S. Patent Number 7,316,903)

The present invention relates, e.g., to a method of detecting a mismatch in a double stranded nucleic acid target, comprising (a) contacting the target with (i) a Mu-end nucleic acid, and (ii) a phage Mu transposase, under conditions effective for the Mu-end nucleic acid to transpose into the target at about the site of a mismatch, if the target comprises a mismatch, and (b) detecting transposition of the Mu-end DNA into the target, wherein transposition of the Mu-end nucleic acid into the target at a predominant site indicates the presence of a mismatch at that site.

Resource Details

https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7316903

tao@niddk.nih.gov

Mizuuchi, Kiyoshi

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Last Reviewed June 2024